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C0493

Sigma-Aldrich

Anti-Calcium Sensing Receptor antibody, Mouse monoclonal

clone HL1499 (6D4-3F4-6C4), purified from hybridoma cell culture

Synonym(s):

Anti-CAR, Anti-EIG8, Anti-FHH, Anti-FIH, Anti-GPRC2A, Anti-HHC, Anti-HHC1, Anti-HYPOC1, Anti-NSHPT, Anti-PCAR1, Anti-hCasR

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About This Item

MDL number:
MFCD02262635
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HL1499 (6D4-3F4-6C4), monoclonal

form

buffered aqueous solution

species reactivity

rabbit, rat, mouse, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 3-6 μg/mL using total extract from rat kidney

isotype

IgG1

UniProt accession no.

P41180

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASR(846)
mouse ... Casr(12374)
rat ... Casr(24247)

Related Categories

General description

The calcium sensing receptor (CASR) gene with 11 exons is mapped to human chromosome 3q13.33-q21.1. CASR is a member of the C family of G protein–coupled receptors and is mainly localized in the parathyroid gland and the thick ascending limb of the Henle loop. CASR is a 1078 amino acid protein.

Immunogen

peptide corresponding to amino-acids 15-29 at the extracellular N-terminus of calcium sensing receptor.

Application

Monoclonal Anti-Calcium Sensing Receptor antibody produced in mouse is suitable for the following applications:
  • Indirect ELISA
  • Immunoblotting
  • Immunocytochemistry
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Western blotting at a concentration of 3-6μg/mL using total extract from rat kidney
Monoclonal anti-calcium sensing receptor antibody produced in mouse has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Calcium sensing receptor (CASR) regulates cell activity according to the extracellular calcium concentration. It also plays a major role in controlling parathyroid hormone (PTH) secretion. Thus, this protein can be considered as a potent target for therapeutic agents designed to modify parathyroid gland function. CASR has a role in key signal transduction pathways in several tissues such as parathyroid gland, kidney, gastrointestinal tract, placenta, pancreas and brain. Mutation in the gene is associated with autosomal dominant hypocalcemic, hypercalciuria and Bartter syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Increased Calcium-Sensing Receptor Immunoreactivity in the Hippocampus of a Triple Transgenic Mouse Model of Alzheimer's Disease.
Emanuela G, et al.
Frontiers in Neuroscience, 2017-2017 null
Calcium-Sensing Receptors
Goodman WG, et al.
Seminars in Nephrology, 24, 17-24 (2004)
Julio Cortijo et al.
Chemico-biological interactions, 183(1), 25-33 (2009-09-29)
Environmental exposure to nickel is associated to respiratory disorders and potential toxicity in the lung but molecular mechanisms remain incompletely explored. The extracellular Ca(2+)-sensing receptor (CaSR) is widely distributed and may be activated by divalent cations. In this study, we
Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells.
Catia V V, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1853(10), 2621-2628 (2015)
Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells
Vaz CV, et al.
Biochimica et Biophysica Acta, 1853, 2621-2628 (2015)
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