C0954
Chondroitinase C from Flavobacterium heparinum
lyophilized powder, ≥200 units/mg solid
Synonym(s):
Chondroitin C lyase
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About This Item
Recommended Products
conjugate
(Glucosaminoglycan)
Quality Level
form
lyophilized powder
specific activity
≥200 units/mg solid
storage temp.
−20°C
Related Categories
General description
Enzyme Commission (EC) Number 4.2.2.x
Biochem/physiol Actions
Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.
Unit Definition
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Y M Michelacci et al.
The Journal of biological chemistry, 251(4), 1154-1158 (1976-02-25)
A chondroitinase that acts upon chondroitin sulfate C and hyaluronic acid was isolated from Flavobacterium heparinum. This enzyme was seperated from constitutional chondroitinase AC and an induced chondroitinase B also present in extracts of F. heparinum previously grown in the
Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B.
N Ototani et al.
Carbohydrate research, 70(2), 295-306 (1979-05-01)
Chondroitinase B and chondroitinase C were separated from an extract of Flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. Chondroitinase C was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. The latter two activities
Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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