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C1427

Sigma-Aldrich

Cytochrome b5 human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

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About This Item

MDL number:
MFCD02687283
UNSPSC Code:
12161501
NACRES:
NA.47

biological source

human

Quality Level

200

recombinant

expressed in E. coli (histidine-tagged)

assay

≥90% (SDS-PAGE)

form

solution

mol wt

16 kDa

technique(s)

inhibition assay: suitable

UniProt accession no.

P00167

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYB5A(1528)

General description

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Biochem/physiol Actions

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.
Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Physical form

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Analysis Note

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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Melissa R Pitman et al.
Scientific reports, 12(1), 454-454 (2022-01-12)
Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents that selectively and specifically target each of the S1P receptors have
Human adrenal cells that express both beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production
Nakamura Y, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 123(3-5), 122-126 (2011)
CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
Stark K, et al.
Arthritis Research & Therapy, 17(1), 56-56 (2015)

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