C2273
Carbonic Anhydrase from bovine erythrocytes
For use as a marker in SDS-PAGE
Synonym(s):
Carbonate Dehydratase, Carbonate Hydrolyase
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About This Item
Recommended Products
biological source
bovine erythrocytes
Quality Level
form
powder
mol wt
~29 kDa
packaging
vial of 5 mg
storage temp.
2-8°C
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General description
Carbonic Anhydrase is a metalloenzyme with zinc. The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).
Application
Carbonic Anhydrase has been used:
- as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples
- as a molecular marker in gel filtration chromatography
- as a standard protein to test the recovery of CMA/20 microdialysis probe
Biochem/physiol Actions
Carbonic anhydrase is essential for the reversible hydration of carbon dioxide. It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways. Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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