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C-39315

Sigma-Aldrich

Fibroblast Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Synonym(s):

Recommended with C-23210

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

form

aqueous solution

packaging

pkg of 1 vial

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Fibroblast Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-23210

Application

Fibroblast Growth Medium is a serum-free medium optimized for the cultivation of neonatal and juvenile fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), juvenile foreskin

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Fibroblast Growth Medium are subjected to comprehensive quality control tests using primary human fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Basic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Storage and Stability

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

recommended

Product No.
Description
Pricing

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Articles

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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