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C-39366

Sigma-Aldrich

Skeletal Muscle Differentiation Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Synonym(s):

Recommended with C-23260

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

aqueous solution

packaging

pkg of 1 vial

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Skeletal Muscle Cell Differentiation Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-23260

Application

The Skeletal Muscle Cell Differentiation Medium is a serum-free medium developed to induce the fusion of skeletal muscle cells to myotubes with typical multinucleated syncytia. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)

Quality

All lots of PromoCell Skeletal Muscle Cell Differentiation Medium is tested for the capacity to fully induce the differentiation of SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Insulin (recombinant human): 10 µg / ml

Storage and Stability

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

recommended

Product No.
Description
Pricing

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary Human Skeletal Muscle Cells and Growth Media

Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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