Skip to Content
MilliporeSigma
All Photos(1)

Documents

C-41210

Sigma-Aldrich

DetachKit

3 x 125 ml

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207

packaging

pkg of 3 × 125 ml

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

DetachKit-125

Application

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

Components

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

Quality

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

Subculture Routine

Click here for more information.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Primary Human Pericytes and Pericyte Growth Media

Primary human pericytes and cell culture media optimized for the in vitro cultivation of human pericytes from all tissues. Protocols for handling, cell passaging, media, and product use.

Primary Human Chondrocytes and Chondrocyte Growth Media

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

Mammary Epithelial Cell Growth Medium

Serum-free Mammary Epithelial Cell Growth Media optimized for the in vitro cultivation of epithelial cells from the mammary gland. Protocols for handling, cell passaging, media, and product use.

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service