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C4951

Sigma-Aldrich

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

Synonym(s):

Cholesterol–methyl-β-cyclodextrin

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.75

biological source

ovine wool

Quality Level

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 300 mg/mL

shipped in

ambient

storage temp.

−20°C

Application

Cholesterol-Water Soluble has been used:
  • in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
  • in cytotoxicity assay
  • for the incubation of CD4+ T cells with Helicobacter pylori
  • to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
  • to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
  • as a supplement for growth of bacterial culture
  • to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
  • to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
  • for reversible inhibition of proteinase K

Packaging

Package size based on cholesterol

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.

Preparation Note

Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methyl-β-cyclodextrin

Sigma-Aldrich

M7439

Methyl-β-cyclodextrin

Cholesterol United States Pharmacopeia (USP) Reference Standard

USP

1131960

Cholesterol

25-Hydroxycholesterol ≥98%

Sigma-Aldrich

H1015

25-Hydroxycholesterol

Sandoz 58-035 >98% (HPLC), powder

Sigma-Aldrich

S9318

Sandoz 58-035

ATR-101, a selective and potent inhibitor of Acyl-CoA acyltransferase 1, induces apoptosis in H295R adrenocortical cells and in the adrenal cortex of dogs
LaPensee CR, et al.
Endocrinology, 157(5), 1775-1788 (2016)
Functional characteristics of Lactobacillus spp. from traditional Maasai fermented milk products in Kenya
Mathara J, et al.
International Journal of Food Microbiology, 126(1-2), 57-64 (2008)
Barbora Melkes et al.
Naunyn-Schmiedeberg's archives of pharmacology, 389(12), 1289-1300 (2016-09-08)
There are some indications that biased μ-opioid ligands may diversely affect μ-opioid receptor (MOR) properties. Here, we used confocal fluorescence recovery after photobleaching (FRAP) to study the regulation by different MOR agonists of receptor movement within the plasma membrane of
Cholesterol influences potassium currents in inner hair cells isolated from guinea pig cochlea
Kimitsuki T
Auris, Nasus, Larynx, 44(1), 46-51 (2017)
Cholesterol does not affect the toxicity of amyloid beta fragment but mimics its effect on MTT formazan exocytosis in cultured rat hippocampal neurons
Abe K and Saito H
Neuroscience Research, 35(3), 165-174 (1999)

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