C6745
Collagen Type IV from human cell culture
Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
About This Item
Recommended Products
biological source
human cell culture
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
170—180 kDa
packaging
pkg of 1 mL
concentration
0.3 mg/mL
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
impurities
HBSAG, none detected
HCV, none detected
HIV-1/HIV-2, none detected
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Laminin
Peptide Source: Nidogen
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... COL4A1(1282)
Related Categories
General description
Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.
Application
Biochem/physiol Actions
Features and Benefits
- This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
- The Collagen Type IV is then purified biochemically.
- It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
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