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C7354

Sigma-Aldrich

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CALB, Anti-D-28K

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About This Item

MDL number:
MFCD00164553
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 28 kDa

species reactivity

rat, chicken, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections
microarray: suitable
western blot: 1:1,000 using recombinant rat calbindin-D-28K

UniProt accession no.

P05937

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALB1(793)
mouse ... Calb1(12307)
rat ... Calb1(83839)

Related Categories

General description

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28 kDa calcium binding protein, with broad tissue distribution. Itbelongs , to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.

Application

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit has been used in:
  • immunocytochemistry
  • western blotting
  • microarray
  • immunohistochemistry

Biochem/physiol Actions

Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.
Calbindin-D-28K is an important neuroanatomical marker. Despite its broad tissue distribution, it exhibits a cell-type-specific expression pattern. It has been immunocytochemically localized in selected cells in hippocampus, where it is thought to buffer intracellular calcium, or transport intramembranous calcium.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Analysis Note

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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hcodes

H412

pcodes

P273

Hazard Classifications

Aquatic Chronic 3

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Distribution of and steroid hormone effects on calbindin-D9k in the immature rat brain
Park SY, et al.
Brain Research Bulletin, 152, 225-235 (2019)
Subpopulations of neurons expressing parvalbumin in the human amygdala
Pantazopoulos H, et al.
The Journal of Comparative Neurology, 496(5), 706-722 (2006)
Janelle M P Pakan et al.
The Journal of comparative neurology, 501(4), 619-630 (2007-02-06)
Purkinje cells in the cerebellum express the antigen zebrin II (aldolase C) in many vertebrates. In mammals, zebrin is expressed in a parasagittal fashion, with alternating immunopositive and immunonegative stripes. Whether a similar pattern is expressed in birds is unknown.
beta-Catenin is critical for cerebellar foliation and lamination
Wen J, et al.
Testing, 8(5), e64451-e64451 (2013)
Altered hippocampal expression of calbindin-D-28k and calretinin in GABAB (1)-deficient mice
Ruttimann E, et al.
Biochemical Pharmacology, 68(8), 1613-1620 (2004)
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