Skip to Content
MilliporeSigma
All Photos(2)

Documents

C9281

Sigma-Aldrich

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

Synonym(s):

CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

CAS Number:
9026-00-0
Enzyme Commission number:
3.1.1.13 (BRENDA, IUBMB)
EC Number:
232-808-6
MDL number:
MFCD00071052
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Pseudomonas fluorescens

Quality Level

200

assay

10-30% (TCA-Biuret)

form

lyophilized powder

specific activity

≥10,000 units/g protein

mol wt

~129 kDa

composition

Protein, ~20%

storage condition

under inert gas (argon)

technique(s)

cell based assay: suitable

color

tan to brown

pH

7-9

solubility

0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Research area: Cell Signaling

Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).

Application

Cholesterol esterase from Pseudomonas fluorescens has been used in:
  • in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
  • a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
  • an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
  • for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
  • to hydrolyze native cholesterol ester (CE) during ​filipin staining for detection of CE within the retinal frozen sections
This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..

Other Notes

Contains potassium phosphate and TRITON® X-100.

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Analysis Note

Protein determined by biuret.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 9

1 of 9

trans-β-Apo-8′-carotenal ≥96.0% (UV)

Sigma-Aldrich

10810

trans-β-Apo-8′-carotenal

all-trans-Astaxanthin analytical standard

Supelco

41659

all-trans-Astaxanthin

Melengestrol acetate VETRANAL®, analytical standard

Supelco

33998

Melengestrol acetate

Sodium sulfate decahydrate ACS reagent, ≥99.0%

Sigma-Aldrich

403008

Sodium sulfate decahydrate

Sigma-Aldrich

Sigma-Aldrich

62309

Lipase from Pseudomonas cepacia

USP

USP

1044200

Astaxanthin (synthetic)

Sigma-Aldrich

Sigma-Aldrich

62305

Lipase from Rhizopus oryzae

Phospholipase A2 from honey bee venom (Apis mellifera) salt-free, lyophilized powder, 600-2400 units/mg protein

Sigma-Aldrich

P9279

Phospholipase A2 from honey bee venom (Apis mellifera)

USP

USP

1044210

Astaxanthin esters from Haematococcus pluvialis

Jounghyun H Lee et al.
Advanced biology, 5(4), e2000428-e2000428 (2021-04-15)
Atherosclerosis begins with the accumulation of cholesterol-carrying lipoproteins on blood vessel walls and progresses to endothelial cell dysfunction, monocyte adhesion, and foam cell formation. Endothelialized tissue-engineered blood vessels (TEBVs) have previously been fabricated to recapitulate artery functionalities, including vasoconstriction, vasodilation
Carolina Espinosa Álvarez et al.
Molecules (Basel, Switzerland), 25(24) (2020-12-30)
Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects
David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Peter E Thelwall et al.
Journal of hepatology, 59(3), 543-549 (2013-04-30)
Lysosomal Acid Lipase (LAL) deficiency is a rare metabolic storage disease, caused by a marked reduction in activity of LAL, which leads to accumulation of cholesteryl esters (CE) and triglycerides (TG) in lysosomes in many tissues. We used (1)H magnetic
Stuart A Scott et al.
Hepatology (Baltimore, Md.), 58(3), 958-965 (2013-02-21)
Cholesteryl ester storage disease (CESD) and Wolman disease are autosomal recessive later-onset and severe infantile disorders, respectively, which result from the deficient activity of lysosomal acid lipase (LAL). LAL is encoded by LIPA (10q23.31) and the most common mutation associated
View All Related Papers

Articles

Cholesterol Esterification

Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service