Recommended Products
product name
RCP, 00031626
biological source
rat brain
growth mode
Adherent
karyotype
Not specified
morphology
Endothelial
products
Not specified
receptors
Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Rat Choroid Plexus
Cell Line Description
The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.
Application
Toxicology, cellular signalling and pharmacology studies
Culture Medium
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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