KARPAS 299
6072604, human lymph, Lymphoblastoid
Synonym(s):
K299 Cells, KARPAS299 Cells
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About This Item
UNSPSC Code:
41106514
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product name
KARPAS 299, 06072604
biological source
human lymph
growth mode
Suspension
karyotype
Hypodiploid
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Non-Hodgkin′s Ki-positive Large Cell Lymphoma
Cell Line Description
A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin′s lymphoma in 1986.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Subculture Routine
Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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