CHP-134
06122002
Synonym(s):
CHP134 Cells, Children′s Hospital of Philadelphia-134 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human nerve (neuroblastoma)
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human neuroblastoma tumour mass of left adrenal gland
Cell Line Description
A neuroblastoma cell line established from a tumour of the left adrenal area of an 18-month-old male patient. The patient was previously treated with vincristine, cyclophosphamide, dimethyltriazenoimidazole carboxamide, adriamycin, and irradiation. The cells grow as adherent colonies consisting of neuroblast-like cells forming dense mounds. Individual cells radiate from the mounds which reach confluency after routine passaging for several months. This cell line has been reported to have tyrosine hydroxylase activity.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 8,11
D16S539: 11,13
D5S818: 11,12
D7S820: 9
THO1: 9,9.3
TPOX: 8
vWA: 18,19
CSF1PO: 9,13
D13S317: 8,11
D16S539: 11,13
D5S818: 11,12
D7S820: 9
THO1: 9,9.3
TPOX: 8
vWA: 18,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split confluent cultures 1:2 to 1:10 every 2-3 days, use gentle agitation to dislodge cells, trypsin/EDTA should not be necessary as cells only adhere loosely; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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