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Hep 3B Cell Line human

86062703, human liver, Epithelial

Synonym(s):

HEP3B217 Cells, Hep 3B2.1-7 Cells, Hep3B Cells

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About This Item

UNSPSC Code:
41106514

product name

Hep 3B Cell Line human, from human liver, 86062703

biological source

human liver

growth mode

Adherent

karyotype

2n = 46, modal no. 60

morphology

Epithelial

products

Alpha-foetoproteins, Hepatitis B Surface Antigen (HBSAg), albumin, transferrin, fibrinogen, complement (C3), alpha-2-macroglobulins

receptors

Not specified

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Description

Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17

Culture Medium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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