LS174T
87060401
Synonym(s):
LS-174-T Cells, LS-174T Cells, LS174 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human colon
growth mode
Adherent
karyotype
Modal no. 45 (2n
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian colon adenocarcinoma
Cell Line Description
A trypsinised variant of LS180 (Sigma Catalogue number. 87021202), both lines being derived from a 58 year old female with Dukes type B adenocarcinoma of the colon. The cells have microvilli and intracytoplasmic vacuoles. The cells are tumorigenic in nude mice.
Application
LS174T is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to study the interaction of enterohaemorrhagic Escherichia coli (EHEC) with the intestinal mucus layer produced by LS174T cells.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C May take up to 7 days for cells to attach. Cells grow in islands and tend to pile on top of each other. Some cells ma
Other Notes
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