A375 Cell Line human
88113005
Synonym(s):
A-375 Cells, A375-MEL Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human skin
description
Human malignant melanoma
growth mode
Adherent
karyotype
Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human malignant melanoma
Cell Line Description
Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.
Application
A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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