Recommended Products
product name
NB4 1A3, 89121405
biological source
mouse nerve
growth mode
Adherent
karyotype
Unstable
morphology
Neuroblast
products
Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse C-1300 Neuroblastoma
Cell Line Description
Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.
Application
Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus
Culture Medium
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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