H69
91091802, human lung, Not specified
Synonym(s):
H69 Cell Line
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About This Item
UNSPSC Code:
41106514
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product name
H69, 91091802
biological source
human lung
growth mode
Aggregates in suspension
karyotype
Aneuploid
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian lung small cell carcinoma
Cell Line Description
This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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