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product name
C2-Rev 7, 94101907
biological source
rat liver
growth mode
Adherent
karyotype
Hyperdiploid, 51 chromosomes
morphology
Epithelial
products
Albumin and hepatocyte markers
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Rat hepatoma [HGPRT-]
Cell Line Description
C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.
Application
Gene expression studies.
Culture Medium
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm2 dish.
Other Notes
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Certificates of Analysis (COA)
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