PNT2 Cell Line human
Normal prostate epithelium immortalized with SV40., 95012613
Synonym(s):
PNT-2 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human prostate
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human prostate normal, immortalised with SV40
Cell Line Description
Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.
Application
Biology of prostate cells, model of differentiated prostate epithelial cells.
PNT2 cell line has been used:
- to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
- to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
- to study the expression of soluble adenylyl cyclase (sAC) in the cells
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17
Culture Medium
RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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