OE21 Cell Line human
96062201, human esophagus, Epithelial
Synonym(s):
JROECL 21 Cells, OE 21 Cells
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About This Item
UNSPSC Code:
41106514
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product name
OE21 Cell Line human, 96062201, Caucasian oesophageal squamous cell carcinoma
biological source
human esophagus
growth mode
Adherent
karyotype
Aneuploid
morphology
Epithelial
products
Not specified
receptors
MHC class I
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian oesophageal squamous cell carcinoma
Cell Line Description
OE21, also known as JROECL21, was established in 1993 from a squamous carcinoma of mid oesophagus of a 74 year-old male patient. The tumour was identified as pathological stage IIA(UICC) and showed moderate differentiation. HLA-A,-B and -C (MHC class I) are expressed constitutively, expression of ICAM-1 can be induced by treatment with interferon-gamma. OE21 cells express epithelial cytokeratins and are tumourigenic in nude mice.
Application
Study of oesophageal cancer
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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