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CS0740

Sigma-Aldrich

Acid phosphatase Assay Kit

1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)

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About This Item

UNSPSC Code:
12161503
eCl@ss:
42010102
NACRES:
NA.84

Quality Level

200

usage

 kit sufficient for 1,000 assays (multiwell plates)
 kit sufficient for 100 assays (tubes)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)

Related Categories

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Biochem/physiol Actions

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets
  • Citrate buffer solution, 0.09 M pH 4.8 100 mL
  • p-Nitrophenol Standard solution 10 mM 1 mL
  • Acid phosphatase control enzyme .2 mL

pictograms

Health hazard
GHS08

signalword

Warning

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Yuan Li et al.
The Journal of cell biology, 215(2), 167-185 (2016-11-05)
Lysosomes degrade macromolecules and recycle metabolites as well as being involved in diverse processes that regulate cellular homeostasis. The lysosome is limited by a single phospholipid bilayer that forms a barrier to separate the potent luminal hydrolases from other cellular
Henning Staedt et al.
Head & face medicine, 16(1), 35-35 (2020-12-09)
The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Eight weeks after formation of a lateral full-thickness perforating bone defect in the
Cheng-Yen Lu et al.
Age (Dordrecht, Netherlands), 37(2), 33-33 (2015-04-13)
Ambient temperature reduction (ATR) can extend the lifespan of organisms, but the underlying mechanism is poorly understood. In this study, cellular degradation activity was evaluated in the muscle of an annual fish (Nothobranchius rachovii) reared under high (30 °C), moderate (25 °C)
Hua Zhao et al.
Journal of neuroscience methods, 217(1-2), 67-74 (2013-04-24)
Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g.
Cameron G McCarthy et al.
American journal of physiology. Heart and circulatory physiology, 317(5), H1013-H1027 (2019-08-31)
Insufficient autophagy has been proposed as a mechanism of cellular aging, as this leads to the accumulation of dysfunctional macromolecules and organelles. Premature vascular aging occurs in hypertension. In fact, many factors that contribute to the deterioration of vascular function
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Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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