D8037
Driselase™ Basidiomycetes sp.
BioReagent, suitable for plant cell culture
Synonym(s):
Driselase™ from Basidiomycetes sp.
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About This Item
Recommended Products
product line
BioReagent
Quality Level
form
powder
composition
Protein, ≥10% biuret
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
−20°C
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Application
Driselase™ Basidiomycetes sp. has been used:
- in spheroplast preparation from Coccomyxa cells
- in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
- for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue
Biochem/physiol Actions
Driselase™ is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.
Other Notes
Crude powder containing laminarinase, xylanase and cellulase.
Legal Information
Driselase is a trademark of ASKA Animal Health Co. Ltd.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The retention times of 10 oligosaccharides, generated from the xyloglucans of Rosa and Tropaeolum by the action of Trichoderma cellulase, and of 17 related carbohydrates, in h.p.l.c. on an amino-substituted silica (Amino-Spheri-5) depended largely on the number of hydroxyl groups
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Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
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Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
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