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D8281

Sigma-Aldrich

Anti-Desmin antibody produced in rabbit

enhanced validation

whole antiserum

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About This Item

MDL number:
MFCD00162258
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, bovine, human, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:20 using cultured cells
indirect immunofluorescence: 1:20 using human skeletal muscle sections
western blot: 1:100 using animal or human skeletal muscle.

UniProt accession no.

P17661

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DES(1674)
mouse ... Des(13346)

Related Categories

General description

Desmin is an intermediate filament protein found mainly in skeletal, cardiac, and smooth muscle cells. It acts as a marker for tumor origin. It also facilitates mechanical support for muscles, mitochondrial localization, myofibrillogenesis, gene expression regulation, and intracellular signalling. Anti-desmin antibody can be used for immunocytochemical localization of intermediate filaments in all types of muscle cells. It can also be used to stain a wide desmin band of 50,000 to 55,000 molecular weight using immunoblotting assays. Rabbit anti-desmin antibody reacts specifically with desmin in bovine, mouse, chicken and human.
Desmin is mapped to human chromosome 2q35. The desmin protein comprises of a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. In smooth and striated muscles, desmin is localized in the periphery of Z-disk.

Immunogen

desmin from chicken gizzard

Application

Anti-Desmin antibody produced in rabbit has been used in:
  • immunohistochemistry of zebrafish intestines sections
  • immunodetection in fish embryo samples
  • immunolabeling of myofibers

Anti-desmin antibody can be used in immunocytochemistry and western immunoblotting. It can also be used in indirect immunofluorescence.

Biochem/physiol Actions

Desmin plays a crucial role in providing mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Missense mutations in desmin gene leads to hypertrophic cardiomyopathy and systemic myopathy. Mutations in desmin gene impairs the stability of intermediary filaments in cardiomyocytes, contributing to pathophysiology of cardiac diseases.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chong YC, et al.
Oncogene (2018)
Functional characterization of the novel DES mutation p. L136P associated with dilated cardiomyopathy reveals a dominant filament assembly defect.
Brodehl A, et al.
Journal of Molecular and Cellular Cardiology, 91(1), 207-214 (2016)
Myogenin promotes myocyte fusion to balance fibre number and size.
Ganassi M, et al.
Nature Communications, 9(1), 4232-4232 (2018)
S Vogel et al.
Journal of lipid research, 41(6), 882-893 (2000-05-29)
Hepatocytes and hepatic stellate cells play important roles in retinoid storage and metabolism. Hepatocytes process postprandial retinyl esters and are responsible for secretion of retinol bound to retinol-binding protein (RBP) to maintain plasma retinol levels. Stellate cells are the body's
Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering.
Maffioletti SM, et al.
Cell Reports, 23(3), 899-908 (2018)
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