E2531
Anti-Erythropoietin antibody produced in rabbit
IgG fraction of antiserum, lyophilized powder
Synonym(s):
Anti-Epo
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-15 μg/mL
neutralization: suitable
western blot: 1 μg/mL
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EPO(2056)
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General description
Epo is mainly produced by liver cells in fetal stages, by kidneys under hypoxic conditions; liver and brain also secrete Epo in less amounts. Anti-Erythropoietin antibody specifically reacts with human Erythropoietin. The EPO gene is mapped to human chromosome 7q22.1.
Immunogen
recombinant human erythropoietin expressed in CHO cells.
Application
Anti- Erythropoietin antibody may be used for immunoblotting at a working concentration of 1 μg/mL. For immunohistochemistry, a working concentration of 5-15 μg/mL may be used. The antibody is also suitable for neutralization reactions. Erythropoietin antibody has been used for ELISA in the extracts of leaves of tobacco plant in which recombinant human erythropoietin was expressed. The antibody has also been used for immunoaffinity chromatography to separate the leaf extracts of tobacco expressing recombinant human erythropoietin.
Biochem/physiol Actions
Erythropoietin (Epo) is a pleotropic cytokine that promotes erythropoiesis or proliferation and differentiation of red blood cell precursors. The red blood cell production is stimulated when Epo binds and activates the Epo receptor (EpoR) dimer. EpoR directly regulates the signalling by Jak2, PI3K, NF-κB, STAT5A and STAT5B. Epo also inhibits apoptosis of erythroid progenitors by downregulating Fas (tumor necrosis factor receptor superfamily member 6) and FasL (Fas ligand). The expression of Epo is under the control of the transcription factor hypoxia inducible factor (HIF). Erythropoietin exhibits disease-modifying effects in infectious diseases and chronic inflammatory disorders Anti-Erythropoietin antibody specifically reacts with human Erythropoietin.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Molecular definition of a narrow interval at 7q22. 1 associated with myelodysplasia.
Johnson E J, et al.
Blood, 87(9), 3579-3586 (1996)
Andrew J Conley et al.
Biotechnology and bioengineering, 103(3), 562-573 (2009-03-07)
The demand for recombinant proteins for medical and industrial use is expanding rapidly and plants are now recognized as an efficient, inexpensive means of production. Although the accumulation of recombinant proteins in transgenic plants can be low, we have previously
Andrew J Conley et al.
Plant biotechnology journal, 7(2), 183-199 (2008-12-06)
Human erythropoietin (EPO) is a pleiotropic cytokine with remarkable tissue-protective activities in addition to its well-established role in red blood cell production. Unfortunately, conventional mammalian cell cultures are unlikely to meet the anticipated market demands for recombinant EPO because of
Farooqahmed S Kittur et al.
Plant cell reports, 31(7), 1233-1243 (2012-03-01)
Erythropoietin (EPO) is a glycoprotein hormone that displays both hematopoietic and tissue-protective functions by binding to two distinct receptors. Recombinant human EPO (rhuEPO) is widely used for the treatment of anemia, but its use for tissue protection is limited because
Wolfgang Jelkmann
The Journal of physiology, 589(Pt 6), 1251-1258 (2010-11-17)
The hormone erythropoietin (Epo) maintains red blood cell mass by promoting the survival, proliferation and differentiation of erythrocytic progenitors. Circulating Epo originates mainly from fibroblasts in the renal cortex. Epo production is controlled at the transcriptional level. Hypoxia attenuates the
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