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E3906

Sigma-Aldrich

Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-EEA1, Anti-Endosome-associated Protein p162, Anti-Zinc Finger FYVE Domain-containing Protein 2

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About This Item

MDL number:
MFCD01633310
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~160 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK cells
western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of human HeLa cells
western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of mouse NIH3T3 cells

UniProt accession no.

Q15075

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EEA1(8411)
mouse ... Eea1(216238)
rat ... Eea1(314764)

General description

Anti-Early Endosomal Antigen 1 (C-terminal), is produced in rabbit using a synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to keyhole limpet hemocyanin (KLH), as immunogen. Early Endosomal Antigen 1 (EEA1) is a 162 kDa autoantigen, which encodes for around 1400 amino acid proteins. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins

Immunogen

synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit has been used in:
  • fluorescent staining
  • confocal laser scanning microscopy
  • immunoprecipitation
  • indirect immunofluorescence microscopy
  • fluorescence microscopy
  • immunoblotting
  • immunofluorescence.

Biochem/physiol Actions

Anti- Early Endosomal Antigen 1 (EEA1) may be used as an early endosome marker. The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. It is associated with subacute systemic lupus erythematosus that specifically localized to early endosomes.
The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic side of early endosomes. EEA1 controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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