E9034
Phosphodiesterase 5A1 human
recombinant, expressed in Sf9 cells
Synonym(s):
3′,5′-cyclic-GMP phosphodiesterase, PDE 5A1
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About This Item
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recombinant
expressed in Sf9 cells
Quality Level
form
solution
specific activity
≥2,000 unit/μg protein
mol wt
126 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE5A(8654)
General description
Research area: Cell SignallingPDE5is located in various tissues such as the kidney, brain, lungs, cardiac myocytes,vascular smooth muscle cells, penile corpus cavernosum, platelets, andgastrointestinal tissues.
Biochem/physiol Actions
PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis . Phosphodiesterase (PDE)is an enzyme that is used to break phosphodiester bonds. Phosphodiesterase 5 (PDE5), amolecular target for the treatment of erectile dysfunction and pulmonaryhypertension. PDE5 is used to find novel PDE5 inhibitors. PDE5 specifically targets cyclicguanosine monophosphate (cGMP) generated by the activation of nitric oxide (NO). PDE5 regulates cGMP signalingin the cardiovascular and other tissues. At the genetic level PDE5 isexpressed as various isoforms and undergoes phosphorylation as a post-translationalmodification that results in its activation. PDE5 is degraded through theubiquitin pathway due to its nitrosylation.
Physical properties
N-terminal GST-tagged 126 kDa full-length protein
Unit Definition
One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.
Physical form
Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20 and 3 mM DTT
Legal Information
TWEEN is a registered trademark of Croda International PLC
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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