F0522
Monoclonal Anti-CD3−FITC antibody produced in mouse
clone UCHT-1, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-CD3
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
UCHT-1, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
flow cytometry: 10 μL using 1 × 106 cells
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CD3E(916)
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General description
CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation.
Specificity
Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2
4th Workshop: paper no. T3.2
Immunogen
human thymocytes followed by Sezary T cells.
Application
Monoclonal Anti-CD3-FITC antibody produced in mouse is suitable for flow cytometry (at a dilution of 10μL using 1 × 106 cells). It was also used for ADCC Assay.
Target description
The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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M R J Green et al.
Clinical and experimental immunology, 141(1), 165-173 (2005-06-17)
Natural killer (NK) cell cytotoxic activity and cell frequency, expressed as a percentage of total lymphocytes, have been determined in peripheral blood mononuclear cells from first-degree relatives of patients with systemic lupus erythematosus (SLE), the patients themselves, a group of
Etienne Gagnon et al.
The Journal of experimental medicine, 209(13), 2423-2439 (2012-11-21)
The CD3ε and ζ cytoplasmic domains of the T cell receptor bind to the inner leaflet of the plasma membrane (PM), and a previous nuclear magnetic resonance structure showed that both tyrosines of the CD3ε immunoreceptor tyrosine-based activation motif partition
Antonino Musolino et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 26(11), 1789-1796 (2008-03-19)
The anti-HER-2/neu monoclonal antibody trastuzumab has been shown to engage both activatory (fragment C receptor [Fc gamma R]IIIa; Fc gamma RIIa) and inhibitory (Fc gamma RIIb) antibody receptors and Fc gamma R polymorphisms have been identified that may affect the
Erika Caro-Gomez et al.
Vaccine, 32(39), 4960-4967 (2014-07-22)
The obligately intracellular bacteria Rickettsia infect endothelial cells and cause systemic febrile diseases that are potentially lethal. No vaccines are currently available and current knowledge of the effective immune response is limited. Natural and experimental rickettsial infections provide strong and
Magda De Smedt et al.
Blood, 110(7), 2696-2703 (2007-07-17)
It has been proposed that heterogeneity in natural killer (NK)-cell phenotype and function can be achieved through distinct thymic and bone marrow pathways of NK-cell development. Here, we show a link between Notch signaling and the generation of intracellular CD3epsilon
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