Skip to Content
MilliporeSigma
All Photos(2)

Documents

F1680

Sigma-Aldrich

Anti-Flotillin 2 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ECS, Anti-ESA, Anti-Epidermal surface antigen, Anti-FLOT2, Anti-Reggie-1

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

MDL number:
MFCD00212936
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~48 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HEK-293T cells
western blot: 0.5-1 μg/mL using whole extract of human HeLa cells

UniProt accession no.

Q14254

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FLOT2(2319)
mouse ... Flot2(14252)
rat ... Flot2(83764)

Related Categories

General description

Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillin 2 is more widely distributed, but is absent in skeletal muscle. FLOT2, also called reggie-1, belongs to flotillin protein family integral membrane proteins comprising of prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain).

Immunogen

synthetic peptide corresponding to amino acid residues 415-428 of human flotillin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

Application

Anti-Flotillin 2 (C-terminal) antibody produced in rabbit has been used in:
  • immunofluorescence
  • immunoprecipitation
  • immunoblotting

Biochem/physiol Actions

Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillins are involved in cell adhesion processes and play a role during breast cancer progression. FLOT2 especially plays a functional role in cell-cell adhesion in non-malignant epithelial cells. It may have an essential function in intestinal physiology, especially in the colon. FLOT2 has been implicated in several signaling pathways in tumor cells and can be used as a prognostic biomarker for breast cancer progression.
The prohibitin homology (PHB) domain constitutes a lipid recognition motif which can target proteins to the plasma membrane. The association of flotillin 2 to the plasma membrane depends on palmitoylation and myristolation in a conserved Cys34. Flotillin 2 overexpression is associated with melanoma progression and transformation of SB2 melanoma cells to a highly metastatic line. It is also associated with expression of the thrombin receptor, protease activated receptor 1 (PAR-1), that is implicated in cell growth and metastasis. Flotillin 2 may be used as a marker protein for lipid raft microdomains.

Target description

Flotillin 2 (C-terminal) encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

related product

Product No.
Description
Pricing

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Regulation of cargo transfer between ESCRT-0 and ESCRT-I complexes by flotillin-1 during endosomal sorting of ubiquitinated cargo
Meister M, et al.
Oncogenesis, 6(6), e344-e344 (2017)
Annika Gauss et al.
International journal of medical sciences, 10(10), 1259-1270 (2013-08-29)
The etiopathogenesis of inflammatory bowel diseases (IBD) remains largely unexplained. Flotillins (flotillin-1 and flotillin-2) are ubiquitous proteins which have been linked to inflammation and regeneration. We hypothesized that alterations in the expression of flotillin-2 in enterocytes may be related to
Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells
Possidonio ACB, et al.
PLoS ONE, 9(8), e103990-e103990 (2014)
Immortalized Human hTert/KER-CT Keratinocytes a Model System for Research on Desmosomal Adhesion and Pathogenesis of Pemphigus Vulgaris
Beckert B, et al.
International Journal of Molecular Sciences, 20(13), 3113-3113 (2019)
The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs
Solomon S, et al.
Immunobiology, 205(1), 108-119 (2002)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service