F1757
Fumarase from porcine heart
ammonium sulfate suspension, ≥300 units/mg protein (biuret)
Synonym(s):
L-Malate hydrolyase, Fumarate hydratase
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About This Item
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form
ammonium sulfate suspension
Quality Level
specific activity
≥300 units/mg protein (biuret)
foreign activity
malic dehydrogenase ≤0.1%
storage temp.
2-8°C
General description
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.
Application
Fumarase from porcine heart has been used:
- as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
- for immobilized enzyme based stability assay
- in fumarate hydratase assays in human lung carcinoma cells (A549)
Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.
Biochem/physiol Actions
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.
Unit Definition
One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol
Preparation Note
Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Fumarase: a mitochondrial metabolic enzyme and a cytosolic/nuclear component of the DNA damage response
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Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases.
Miettinen M, et al.
American Journal of Surgical Pathology, 40(12), 1661-1669 (2016)
E V Chernigovskaia et al.
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Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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