F6162
Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal
clone FB-8, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse, Anti-FGF2
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About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FB-8, monoclonal
form
buffered aqueous solution
mol wt
antigen 18 kDa
species reactivity
bovine, human
technique(s)
dot blot: suitable
indirect ELISA: suitable
neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FGF2(2247)
General description
Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the FB-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Fibroblast growth factors (FGFs) are members of a family of polypeptides. The names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). bFGF usually has an apparent mass of about 18 kDa.
Specificity
The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1.
Immunogen
recombinant human FGF2.
Application
Anti-Fibroblast Growth Factor-Basic antibody has been used in in immunolabelling and western blotting. It may also be used in:
- in immunoblotting
- in enzyme linked immunosorbent assay (ELISA)
- in dot blot
- in radio immune assay (RIA)
- in neutralization bioassays
Used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture.
Biochem/physiol Actions
Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis.
Physical form
Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
An intrinsic quality-control mechanism ensures unconventional secretion of fibroblast growth factor 2 in a folded conformation
Torrado L C,et al.
Journal of Cell Science, 122(18), 3322-3329 (2009)
S A Boldin et al.
The Journal of biological chemistry, 275(14), 9905-9909 (2000-04-01)
We have previously shown that ongoing glucosylceramide (GlcCer) synthesis is required for basic fibroblast growth factor (bFGF) and laminin to stimulate axonal growth in cultured hippocampal neurons (Boldin, S., and Futerman, A. H. (1997) J. Neurochem. 68, 882-885). We now
Lucía Cespón Torrado et al.
Journal of cell science, 122(Pt 18), 3322-3329 (2009-08-27)
Fibroblast growth factor 2 (FGF2) is a proangiogenic mitogen that is secreted by an unconventional mechanism, which does not depend on a functional ER-Golgi system. FGF2 is first recruited to the inner leaflet of plasma membranes, in a process that
J Rödel et al.
Infection and immunity, 68(6), 3635-3641 (2000-05-19)
Chlamydia pneumoniae infection has been associated with asthma and atherosclerosis. Smooth muscle cells represent host cells for chlamydiae during chronic infection. In this study we demonstrated that C. pneumoniae infection of human smooth muscle cells in vitro increased production of
Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix
Aktas RG and Kayton RJ
Histochemistry and Cell Biology, 113(3), 227-233 (2000)
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