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F7253

Sigma-Aldrich

Fast Black K Salt hemi(zinc chloride) salt

Dye content, ≥25%, practical grade, powder

Synonym(s):

2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt, Brentamine fast black K salt

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About This Item

Linear Formula:
C14H12N5O4 · 1/2ZnCl4
CAS Number:
Molecular Weight:
417.88
Colour Index Number:
37190
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Fast Black K Salt hemi(zinc chloride) salt, practical grade

grade

practical grade

Quality Level

form

powder

composition

Dye content, ≥25%

color

brown-red

mp

150 °C

solubility

water: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1N=Nc2ccc(cc2)[N+]([O-])=O.COc3cc([N+]#N)c(OC)cc3N=Nc4ccc(cc4)[N+]([O-])=O

InChI

1S/2C14H12N5O4.4ClH.Zn/c2*1-22-13-8-12(14(23-2)7-11(13)16-15)18-17-9-3-5-10(6-4-9)19(20)21;;;;;/h2*3-8H,1-2H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

NZWZSUSPTZUGNN-UHFFFAOYSA-J

General description

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

Application

Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Fast black K salt has various applications as listed below-

  • to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
  • as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
  • for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
  • staining endopeptidase and leucine aminopeptidase activity

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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