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F8399

Sigma-Aldrich

Riboflavin 5′-monophosphate sodium salt hydrate

≥95% (HPLC)

Synonym(s):

FMN

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About This Item

Empirical Formula (Hill Notation):
C17H20N4O9P · xNa+ · yH2O
Molecular Weight:
455.34 (anhydrous free acid basis)
EC Number:
204-988-6
UNSPSC Code:
41106305
eCl@ss:
34058005
NACRES:
NA.51

Quality Level

200

assay

≥95% (HPLC)

form

powder

storage temp.

−20°C

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General description

Riboflavin 5′-monophosphate (FMN) is highly soluble in water. Flavins are considered as blue-light absorbing dyes.

Application

Riboflavin 5′-monophosphate (FMN) is used as a prosthetic group by oxidoreductase (one and two electron transfer) enzymes such as NADH dehydrogenases, nitric oxide synthases, and nitrilotriacetate monooxygenases. Riboflavin 5′-monophosphate is used as a substrate to study the specificity and kinetics of FMN phosphohydrolases. FMN is used to study molecular sensing based gene regulation by riboswitches.
Riboflavin 5′-monophosphate sodium salt hydrate has been used:
  • in the study to examine FMN (flavin mononucleotide) photo-reduction in aqueous solutions
  • to immobilize on titanium oxide modified carbon fibre to determine its electrochemical behavior
  • in flavin identification

Biochem/physiol Actions

Riboflavin 5′-monophosphate (FMN) acts as a cofactor in flavoproteins and possess biological activity in enzymes and photoreceptors.

Preparation Note

Prepared by the enzymatic hydrolysis of flavin adenine dinucleotide.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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USP

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Supelco

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Riboflavin (B2)

Delivery of Riboflavin-5?-Monophosphate Into the Cornea: Can Liposomes Provide Any Enhancement Effects?
Kandzija N and Khutoryanskiy VV
Journal of Pharmaceutical Sciences, 106(10), 3041-3049 (2017)
Electrochemical behaviour of FAD and FMN immobilised on TiO2 modified carbon fibres supported by ATR-IR spectroscopy of FMN on TiO2
Kubota LT, et al.
Bioelectrochemistry and bioenergetics, 47(1), 39-46 (1998)
Photo-induced reduction of flavin mononucleotide in aqueous solutions
Song SH, et al.
Chemical Physics, 332(1), 55-65 (2007)
Induction and catalytic properties of an intracellular NADH-dependent 1, 4-benzoquinone reductase from the brown-rot basidiomycete Gloeophyllum trabeum
Qi W and Jellison J
International Biodeterioration & Biodegradation, 54(1), 53-60 (2004)
Nathan J Baird et al.
RNA biology, 7(3), 328-332 (2010-05-12)
Riboswitches are structured mRNA elements involved in gene regulation that respond to the intracellular concentration of specific small molecules. Binding of their cognate ligand is thought to elicit a global conformational change of the riboswitch, in addition to modulating the
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