F8402
Monoclonal Anti-CD31 (PECAM-1)−FITC antibody produced in mouse
clone WM-59, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-CD31, Anti-PECAM-1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
WM-59, monoclonal
form
buffered aqueous solution
mol wt
antigen 130-140 kDa
species reactivity
human
storage condition
protect from light
technique(s)
flow cytometry: 10 μL using 1 × 106 cells
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... PECAM1(5175)
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General description
CD31 is a single chain integral membrane glycoprotein member of the immunoglobulin superfamily of cell adhesion molecules with molecular mass of 130-140kDa. It has six Ig-like loops in the extracellular domain, a trans-membrane domain including a relatively long cytoplasmic domain. It is expressed abundantly on platelets, myeloid cells, B lymphocytes, certain T-lymphocyte cell subsets, bone marrow precursor cells, NK cells and localized to the intracellular junctions in monolayers of cultured endothelial cells.
Monoclonal Anti-CD31 (PECAM-1) is derived from the WM-59 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025
5th Workshop: code No. P025
Immunogen
human cell line RC-2A, originally derived from myeloid leukemia cells.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Flow cytometry/Cell sorting (1 paper)
Monoclonal Anti-CD31 (PECAM-1)-FITC antibody has been used in
- DAB based-immunohistochemistry
- direct immunofluorescent staining
- fluorescence-activated cell sorting (FACS)
- flow cytometry
Monoclonal Anti-CD31 (PECAM-1)-FITC antibody is suitable for immunostaining of HEPC-CB2 and HEPC-CB.2 cell Lines (hematopoietic cells) and flow cytometry to evaluate the percentage of contaminating endothelia cells in characterization and decidualization of endometrial stromal cells.
Biochem/physiol Actions
CD31 is also known as PECAM-1 (Platelet endothelial cell adhesion molecule-1) binds to glycosaminoglycans (GAG). Endothelial CD31 is phosphorylated on tyrosine and serine residues. CD31 is involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.
CD31 performs in homophilic and heterophilic cell-cell adhesion and cell signalling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix.
Target description
Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Bisphenol A alters-hCG and MIF release by human placenta: an in vitro study to understand the role of endometrial cells
Mannelli C, et al.
Mediators of Inflammation, 2014 (2014)
L Piali et al.
The Journal of cell biology, 130(2), 451-460 (1995-07-01)
To protect the body efficiently from infectious organisms, leukocytes circulate as nonadherent cells in the blood and lymph, and migrate as adherent cells into tissues. Circulating leukocytes in the blood have first to adhere to and then to cross the
Inhibin is a novel paracrine factor for tumor angiogenesis and metastasis
Singh P, et al.
Cancer Research, 78(11), 2978-2989 (2018)
P J Newman
Annals of the New York Academy of Sciences, 714, 165-174 (1994-04-18)
Platelet/Endothelial Cell Adhesion Molecule-1 (PECAM-1) is a 130-kDa integral membrane glycoprotein found on the surface of human platelets and leukocytes, and at the intercellular junctions of endothelial cells. PECAM-1 is a member of the immunoglobulin (Ig) gene superfamily, with six
Platelet/endothelial cell adhesion molecule-1 (CD31)-mediated cellular aggregation involves cell surface glycosaminoglycans.
DeLisser H M, et al.
The Journal of Biological Chemistry, 268(21), 16037-16046 (1993)
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