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G5422

Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

≤1.0 mol % L-α-isomer

Synonym(s):

BGP, Glycerol 2-phosphate disodium salt hydrate

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About This Item

Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
Molecular Weight:
216.04 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.25

biological source

synthetic

Quality Level

form

powder

impurities

≤0.1% Inorganic phosphorus
≤1.0 mol % L-α-isomer

mp

102-104 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
β-glycerophosphate is used in homegenization solutions for muscles extracts following fixation.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Morten Donsmark et al.
The Journal of physiology, 550(Pt 3), 845-854 (2003-06-10)
Intramuscular triacylglycerol is an important energy store and is also related to insulin resistance. The mobilization of fatty acids from this pool is probably regulated by hormone-sensitive lipase (HSL), which has recently been shown to exist in muscle and to
J Douglas et al.
The Journal of applied bacteriology, 56(2), 321-326 (1984-04-01)
An easily prepared medium, originally designed for the cultivation of lactic phages, has been found to have much wider application. Experience in its use over a ten year period with a range of physiologically diverse bacteria, for teaching and research
Yang An et al.
Biochemical and biophysical research communications, 516(2), 551-557 (2019-06-27)
Developing methods for regenerating bones and uncovering the molecular mechanism underlying bone formation have great significance to human health. In the last decade, people have been using adipose-derived stem cells (ADSCs), that are capable of multilineage differentiation, to reconstruct defected
Guoqiang Zhao et al.
Marine pollution bulletin, 149, 110582-110582 (2019-09-25)
Phosphorus (P) pollution can trigger severe marine eutrophication, which further leads to harmful algal blooms, and a deterioration of sea water quality. The P burial and regeneration in offshore sediments can directly affect the eutrophication levels of estuarine and coastal
Lucas R Brun et al.
SpringerPlus, 3, 676-676 (2014-12-19)
The aim of this study was to evaluate the effect of strontium ranelate (SrR) on bone mineral density (BMD) and bone turnover markers after 1 year of treatment. Additionally, the effect of SrR in bisphosphonate-naïve patients (BP-naïve) compared to patients previously

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