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G5791

Sigma-Aldrich

Anti-phospho-Glycogen Synthase Kinase 3α/β (pTyr279/216) antibody produced in rabbit

affinity isolated antibody, aqueous glycerol solution

Synonym(s):

Anti-phospho-GSK α/β (pTyr279/216)

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About This Item

MDL number:
MFCD03455745
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

aqueous glycerol solution

mol wt

antigen 47-51 kDa

species reactivity

human, mouse, rat

technique(s)

dot blot: suitable
indirect ELISA: suitable
western blot: 1:1000 using MCF-7 cell extract

UniProt accession no.

P49840

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr279/pTyr216)

Gene Information

human ... GSK3A(2931)
mouse ... Gsk3a(606496)
rat ... Gsk3a(50686)

General description

GSK-3 functions a serine-threonine kinase and modulates a wide range of cellular activities such as cell signaling, cell cycle, cell growth and differentiation. Mammalian GSK-3 has two isoforms, namely, GSK-3α and GSK-3 β. GSK-3α has been implicated in Alzheimer′s disease, whereas both, GSK-3α and GSK-3β have been implicated in leukemia . Anti-phospho-Glycogen Synthase Kinase 3α/β (p(Tyr279/216)) antibody is specific for phosphorylated forms of GSK-3α (51 kDa) and GSK-3β (47 kDa) that contain a phosphate moiety on tyrosine 279 and 216 respectively. The antibody does not bind to non-tyrosine phosphorylated GSK-3α/β protein. The product recognizes human, mouse and rat GSK-3α/β.

Immunogen

synthetic phosphopeptide derived from the regions of GSK-3α/β that contain tyrosine279/216.

Application

Anti-phospho-Glycogen Synthase Kinase 3α/β (p(Tyr279/216)) antibody is suitable for use in indirect ELISA, dot blot and western blot (1:1000 using MCF-7 cell extract).

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/ml BSA (IgG and protease free), and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emma Y Song et al.
Experimental hematology, 38(10), 908-921 (2010-06-15)
The objective of this study was to investigate the effect of small molecule inhibitors of glycogen synthase kinase-3β (GSK-3β) on leukemia cell growth and survival. Analysis of cytotoxicity and cell proliferation was conducted using the MTS assay, cell-cycle analysis, and
Christopher J Phiel et al.
Nature, 423(6938), 435-439 (2003-05-23)
Alzheimer's disease is associated with increased production and aggregation of amyloid-beta (Abeta) peptides. Abeta peptides are derived from the amyloid precursor protein (APP) by sequential proteolysis, catalysed by the aspartyl protease BACE, followed by presenilin-dependent gamma-secretase cleavage. Presenilin interacts with
Bradley W Doble et al.
Developmental cell, 12(6), 957-971 (2007-06-05)
In mammalian cells, glycogen synthase kinase-3 (GSK-3) exists as two homologs, GSK-3alpha and GSK-3beta, encoded by independent genes, which share similar kinase domains but differ substantially in their termini. Here, we describe the generation of an allelic series of mouse
Kashif Aziz Khan et al.
Molecular and cellular biology, 35(17), 3029-3043 (2015-06-24)
Induction of an antiviral innate immune response relies on pattern recognition receptors, including retinoic acid-inducible gene 1-like receptors (RLR), to detect invading pathogens, resulting in the activation of multiple latent transcription factors, including interferon regulatory factor 3 (IRF3). Upon sensing
Versha Banerji et al.
The Journal of clinical investigation, 122(3), 935-947 (2012-02-14)
Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults. Long-term survival of patients with AML has changed little over the past decade, necessitating the identification and validation of new AML targets. Integration of genomic approaches
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