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G8541

Sigma-Aldrich

Ala-Gln

200 mM, solution, sterile-filtered, Biotechnology Performance Certified

Synonym(s):

Alanyl-glutamine, Glutamine-S

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About This Item

Empirical Formula (Hill Notation):
C8H15N3O4
CAS Number:
39537-23-0
Molecular Weight:
217.22
MDL number:
MFCD00133046
UNSPSC Code:
12352209
PubChem Substance ID:
24895343

grade

Biotechnology Performance Certified

Quality Level

400

sterility

sterile-filtered

form

solution

concentration

200 mM

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

NC(CC[C@H](NC([C@@H](N)C)=O)C(O)=O)=O

InChI

1S/C8H15N3O4/c1-4(9)7(13)11-5(8(14)15)2-3-6(10)12/h4-5H,2-3,9H2,1H3,(H2,10,12)(H,11,13)(H,14,15)/t4-,5-/m0/s1

InChI key

HJCMDXDYPOUFDY-WHFBIAKZSA-N

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General description

Ala-Gln supplement provides a stable form of L-glutamine while in solution.

Application

Ala-Gln has been used as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) media-rich in glucose and pyruvate to culture luciferase-expressing MDA-MB-231-luc tumor cells to measure the bioluminescence signals of cells leaking during intra-bone marrow transplantation (IBMT) from dissected long bones. It has also been used as a DMEM - high glucose media to culture human cell lines.

Other Notes

Contains the dipeptide L-Alanyl-L-glutamine. 200 mM supplied in 0.85% NaCl.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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USP

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SAFC

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A I Gonçalves et al.
Journal of tissue engineering and regenerative medicine, 12(3), 762-774 (2017-06-09)
Cell-based therapies are of particular interest for tendon and ligament regeneration given the low regenerative potential of these tissues. Adipose tissue is an abundant source of stem cells, which may be employed for the healing of tendon lesions. However, human
Daren K Heyland et al.
JPEN. Journal of parenteral and enteral nutrition, 39(4), 401-409 (2014-05-08)
The recent large randomized controlled trial of glutamine and antioxidant supplementation suggested that high-dose glutamine is associated with increased mortality in critically ill patients with multiorgan failure. The objectives of the present analyses were to reevaluate the effect of supplementation
Dieter Fink et al.
PloS one, 14(11), e0224576-e0224576 (2019-11-08)
Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable
Yu-Chen Hou et al.
The Journal of nutritional biochemistry, 24(9), 1555-1563 (2013-03-23)
Regulatory T cells (Tregs) and T helper (Th) 17 cells are two subsets of Th cells with opposing actions which participate in the process of resolving acute lung injury (ALI). Glutamine (Gln) is a nutrient commonly used in nutrition regimens
Marek Bartosovic et al.
Nature biotechnology, 39(7), 825-835 (2021-04-14)
In contrast to single-cell approaches for measuring gene expression and DNA accessibility, single-cell methods for analyzing histone modifications are limited by low sensitivity and throughput. Here, we combine the CUT&Tag technology, developed to measure bulk histone modifications, with droplet-based single-cell
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