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GE17-0440-01

Con-A Sepharose 4B

Cytiva 17-0440-01, pack of 100 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

concanavalin A (con A)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-0440-01

storage condition

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-9

working range

4-6

storage temp.

2-8°C

Related Categories

General description

Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.

Features and Benefits

  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars.
  • Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
  • Con A coupled to Sepharose 4B via cyanogen bromide activation

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Warning

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Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

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Articles

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This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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