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GE17-0717-01

HiTrap® NHS-activated High Performance

Cytiva 17-0717-01, pack of 1 × 5 mL

Synonym(s):

Affinity chromatography column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

N-hydroxysuccinimide (NHS)

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 17-0717-01

storage condition

(100% Isopropanol)

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

ligand dependent

3-12(Ligand dependent)

storage temp.

2-8°C

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General description

HiTrap® NHS activated HP columns are prepacked with NHS activated Sepharose™ High Performance. This high-performance medium is designed for easy coupling of ligands containing primary amines.

HiTrap® NHS-activated HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the medium, provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC™ System.

Features and Benefits

  • For coupling ligands via primary amines.
  • Simple coupling, carried out at close to neutral pH.
  • Delivered in 100% isopropanol to protect the active groups.
  • 10 μmol NHS groups/mL medium.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service