GE17-0756-05
Glutathione Sepharose™ 4B
Cytiva 17-0756-05, pack of 100 mL
Synonym(s):
affinity chromatography resin, GST-tagged proteins purification
About This Item
Recommended Products
ligand
glutathione
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0756-05
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)
Related Categories
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Storage and Stability
Analysis Note
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Certificates of Analysis (COA)
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Articles
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Protocols
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
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