GE17-3712-02
Ni Sepharose™ Excel
Cytiva 17-3712-02, pack of 100 mL
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-3712-02
matrix
highly cross-linked 6% agarose
average diameter
90 μm
cleaning in place
2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
working range
2-13
capacity
≥10 mg binding capacity (histidine-tagged protein)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6- tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
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General description
Application
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Analysis Note
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hcodes
Storage Class
3 - Flammable liquids
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Articles
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Protocols
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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