Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE17-5161-01

HiTrap® SP XL

Cytiva 17-5161-01, pack of 5 × 5 mL

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5161-01

parameter

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiTrap® SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Application

Q Sepharose XL and SP Sepharose XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Features and Benefits

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Storage and Stability

Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 10

1 of 10

HiTrap&#174; Q High Performance Cytiva 17-1153-01, pack of 5 × 1&#160;mL

GE17-1153-01

HiTrap® Q High Performance

HiTrap&#174; Q High Performance Cytiva 17-1154-01, pack of 5 × 5&#160;mL

GE17-1154-01

HiTrap® Q High Performance

HiTrap&#174; Q Fast Flow Cytiva 17-5156-01, pack of 5 × 5&#160;mL

GE17-5156-01

HiTrap® Q Fast Flow

HisTrap&#8482; Fast Flow Crude Cytiva 17-5286-01, pack of 5 × 5&#160;mL

GE17-5286-01

HisTrap Fast Flow Crude

HiTrap&#174; DEAE Fast Flow Cytiva 17-5055-01, pack of 5 × 1&#160;mL

GE17-5055-01

HiTrap® DEAE Fast Flow

HisTrap&#8482; Fast Flow Cytiva 17-5255-01, pack of 5 × 5&#160;mL

GE17-5255-01

HisTrap Fast Flow

HisTrap&#8482; High Performance Cytiva 17-5248-02, pack of 5 × 5&#160;mL

GE17-5248-02

HisTrap High Performance

HiTrap&#174; Desalting Columns Cytiva 17-1408-01, pack of 5 × 5&#160;mL

GE17-1408-01

HiTrap® Desalting Columns

E-64 protease inhibitor

Sigma-Aldrich

E3132

E-64

Articles

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service