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sterility
non-sterile
form
liquid
feature
wetted part: no
packaging
pkg of 1 mL
manufacturer/tradename
Cytiva 17-6004-40
color
Colorless to light yellow
pH range
3-11
solubility
cold water: soluble (Soluble in cold water and hot water)
storage temp.
2-8°C
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General description
Application
IPG buffers are supplied in 1-ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.
Features and Benefits
- For use with Immobiline® DryStrip gels to improve Protein solubility.
- Using Immobiline® DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting Low abundance Proteins.
- Improved IPG buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional Protein spots in high molecular weight and basic pH areas.
- Eliminates background staining.
- Ensures optimal conditions for IEF separation.
Storage and Stability
Analysis Note
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.
This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.
Protocols
Sample Grinding Kit from GE Healthcare is designed to disrupt cell or tissue samples for protein extraction.
Sample Grinding Kit from Cytiva Healthcare is designed to disrupt cell or tissue samples for protein extraction
This page shows how to use the IPGphor fixed-length Strip Holders from Cytiva for gel rehydration in 2-D electrophoresis.
This page describes two-dimensional separation of protein samples with Cytiva products.
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