Recommended Products
ligand
glutathione
material
polypropylene and polyethylene
manufacturer/tradename
Cytiva 28-4055-00
packaging
pkg of 4 x 96-well
size
127.8 mm × 85.5 mm × 30.6 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)
Related Categories
General description
Application
Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrap™ FF is prepacked with Glutathione Sepharose™ 4 Fast Flow, while GST MultiTrap™ 4B is prepacked with Glutathione Sepharose™ 4B.
Features and Benefits
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose™ media for convenience.
- Easy and predictable scale-up using prepacked GSTrap™ FF, GSTPrep™ FF 16/10, and GSTrap™ 4B columns.
Storage and Stability
Analysis Note
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
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This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Protocols
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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