HiTrap^® MabSelect^™ Xtra

ligand

recombinant protein A (E. coli)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 28-4082-60

storage condition

(20% Ehtanol)
(20% Ethanol)

parameter

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked agarose

avg. part. size

75 (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

capacity

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)

suitability

suitable for bioprocess medium

storage temp.

2-8°C