GE28-9031-33
Protein A High Performance MultiTrap™
Cytiva 28-9031-33
Synonym(s):
High Performance Protein A, MultiTrap Protein A
About This Item
Recommended Products
ligand
native protein A
material
polypropylene and polyethylene
feature
wetted part: no
packaging
pkg of 4 ea
manufacturer/tradename
Cytiva 28-9031-33
storage condition
(20% Ethanol)
parameter
30 °C max. temp.
4 °C min. temp.
size
127.8 mm × 85.5 mm × 30.6 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-10
working range
3-9
storage temp.
2-8°C
Related Categories
General description
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Features and Benefits
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, with protocols for classic elution and cross-link method.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
Storage and Stability
Analysis Note
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Certificates of Analysis (COA)
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Articles
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
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