GE28-9187-80
MBPTrap™ HP
Cytiva 28-9187-80, pack of 5 × 5 mL
Synonym(s):
MBP Trap
About This Item
Recommended Products
ligand
dextrin
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-9187-80
storage condition
(20% Ehtanol)
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-13
working range
>7
capacity
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
storage temp.
2-8°C
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General description
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
Features and Benefits
- HiTrap® columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
Storage and Stability
Analysis Note
Other Notes
Legal Information
signalword
WarningDanger
hcodes
Hazard Classifications
Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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Protocols
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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