Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pack of 1 kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 1 kit

manufacturer/tradename

Cytiva 28-9321-71

storage condition

(0.15% Kathon CG)
 (20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

Related Categories

General description

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Features and Benefits

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

Components

The kit contains:

1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SpinTrap is a trademark of Cytiva

Kit Components Also Available Separately

Product No.
Description
SDS

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 8

1 of 8

HisTrap™ High Performance Cytiva 29-0510-21, pack of 1 mL

GE29-0510-21

HisTrap™ High Performance

HiTrap® Hic Selection Kit Cytiva 28-4110-07, pack of 7 × 1 mL

GE28-4110-07

HiTrap® Hic Selection Kit

HisTrap™ High Performance Cytiva 17-5247-01, pack of 5 × 1 mL

GE17-5247-01

HisTrap™ High Performance

HisTrap™ Excel Cytiva 17-3712-06, pack of 5 × 5 mL

GE17-3712-06

HisTrap™ Excel

HIS-Select® Nickel Affinity Gel (1:1 suspension in a 20% ethanol solution)

Millipore

P6611

HIS-Select® Nickel Affinity Gel

cOmplete™ His-Tag Purification Resin suspension, suitable for protein purification, matrix Sepharose-CL 6B

Roche

COHISR-RO

cOmplete™ His-Tag Purification Resin

HisTrap™ Fast Flow Cytiva 17-5319-01, pack of 5 × 1 mL

GE17-5319-01

HisTrap™ Fast Flow

HisTrap™ Fast Flow Cytiva 17-5255-01, pack of 5 × 5 mL

GE17-5255-01

HisTrap™ Fast Flow

Articles

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Protocols

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service