GE28-9440-06
Protein A Mag Sepharose™
Cytiva 28-9440-06, pack of 1 × 500 μL
About This Item
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ligand
native protein A
packaging
pack of 1 × 500 μL
manufacturer/tradename
Cytiva 28-9440-06
storage condition
(20% Ethanol, 20% medium slurry)
parameter
Room temperature temp. range
matrix
paramagnetic, spherical, highly cross-linked agarose particles
capacity
8- 17 mg binding capacity(human IgG/ml gel)
storage temp.
2-8°C
Related Categories
General description
Application
There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.
Protein A Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
Features and Benefits
- Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
Storage and Stability
Analysis Note
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Certificates of Analysis (COA)
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Articles
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
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